ISO/TS TECHNICAL SPECIFICATION 16782 First edition 2016-10-15 Clinical laboratory testing Criteria for acceptable lots of dehydrated Mueller-Hinton agar and broth for antimicrobial susceptibility testing Détermination de la sensibilité aux antibiotiques - Criteres d'acceptabilite pour les lots d'agar déshydraté et de bouillon Mueller- Hinton pour déterminer la sensibilité aux antibiotiques Reference number X ISO/TS 16782:2016(E) ISO International Organization for Standardization @IS02016 IS0/TS 16782:2016(E) COPYRIGHT PROTECTED DOCUMENT IS0 2016, Published in Switzerland All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form written permission. Permission can be requested from either Iso at the address below or Iso's member body in the country of the requester. IsO copyrightoffice Ch. de Blandonnet 8 . CP 401 CH-1214 Vernier, Geneva, Switzerland Tel. +41 22 749 01 11 Fax +41 22 749 09 47 [email protected] www.iso.org Internatinir DrganizationforStandardization No reproduction or networking permited without license from IHS IS0/TS 16782:2016(E) Contents Page Foreword ..iv Introduction. ..V 1 Scope. ..1 2 Normative reference ..1 3 Terms and definitions ..1 4 Requirements for Mueller-Hinton broth .3 4.1 Components of Mueller-Hinton broth .3 4.2 Physical and chemical characteristics. .3 4.2.1 Dehydrated powder or granules .3 4.2.2 Prepared broth medium .4 4.2.3 Cation supplementation and content for MHB 4 4.2.4 Other medium components. 4 4.2.5 Specific adjustments required by the manufacturer 4 4.3 Manufacturers protocol for testing production lots of dehydrated Mueller-Hinton broth...5 4.4 Interpreting the results 5 4.5 Evaluating the results. .6 5 Requirements for Muller-Hinton agar .6 5.1 Components of Mueller-Hinton agar .6 5.2 Physical and chemical characteristics .6 5.2.1 Dehydrated powder or granules .6 5.2.2 Prepared agar medium. 7 5.2.3 Cation supplementation and content for MHA 7 5.2.4 Other medium components. 5.2.5 Specific adjustments required by the manufacturer 5.3 Manufacturer's protocol for testing production lots of dehydrated Mueller-Hinton agar .. 8 5.4 Interpreting the results 8 5.5 Evaluating the results. 10 6 Testing new antimicrobial agents with production lots of dehydrated Mueller- Hinton broth or agar. ..11 Annex A (informative) Mueller-Hinton medium ..12 Annex B (informative) Preparing control cultures .14 Annex C (informative) Suggested data sheet for testing of production lots ..16 Annex D (informative) Label statement ..19 Bibliography .20 ii without license from IHS