化学品　嗜热四膜虫多代繁殖毒性试验 Chemicals— Tetrahymena thermophila multi-generational reproductive test  ICS  13.300 CCS  R 04   中华人民共和国国家标准 GB/T 44482—2024 2024-09-2 9发布 2025-04-0 1实施     国家市场监督管理总局 国家标准化管理委员会 发 布 目　　次 前言   ·····································································································   Ⅲ 1     范围   ··································································································   1 2     规范性引用文件   ······················································································   1 3     术语和定义   ···························································································   1 4     试验原理   ······························································································   1 5     受试物信息   ···························································································   2 6     参比物质   ······························································································   2 7     试验系统   ······························································································   2      7.1     仪器设备   ·························································································   2      7.2     材料和试剂   ······················································································   2      7.3     受试生物的选取   ·················································································   3      7.4     试验溶液   ·························································································   3      7.5     试验前的准备   ····················································································   3      7.6     预试验   ···························································································   3      7.7     正式试验程序   ····················································································   3 8     限度试验   ······························································································   4 9     质量控制   ······························································································   5 10     数据与报告   ··························································································   5      10.1     抑制率的计算   ···················································································   5      10.2     ECx的计算   ······················································································   5      10.3     LOEC和NOEC的计算   ·········································································   5      10.4     试验报告   ························································································   5 附录 A （资料性）　嗜热四膜虫的实验室维护及培养方法实施指南   ···································   7      A.1     概述   ·····························································································   7      A.2     常用培养基   ······················································································   7      A.3     嗜热四膜虫的保种   ··············································································   7      A.4     嗜热四膜虫的培养   ··············································································   8 附录 B （资料性）　嗜热四膜虫不同时长﹘繁殖代际参考表   ············································   9 附录 C （资料性）　嗜热四膜虫暴露试验24 孔板设置推荐方案   ······································   10 附录 D （资料性）　嗜热四膜虫相对细胞活力检测96孔板设置推荐方案   ·····························   11 参考文献   ································································································   12 GB/T 44482—2024     Ⅰ